M-CSF as a therapeutic target in BRAF^V600E melanoma resistant to BRAF inhibitors.

ABSTRACT:

Background

Disseminated BRAF^V600E melanoma responds to BRAF inhibitors (BRAFi) but easily develops resistance with poor prognosis. Secretome plays a pivotal role during tumour progression causing profound effects on therapeutic efficacy. Secreted M-CSF is involved in both cytotoxicity suppression and tumour progression in melanoma. We aimed to analyse the M-CSF contribution in resistant metastatic melanoma to BRAF-targeted therapies.

Methods

Conditioned media from melanoma cells were analysed by citoarray. Viability and migration/invasion assays were performed with paired melanoma cells and tumour growth in xenografted SCID mice. We evaluated the impact of M-CSF plasma levels with clinical prognosis from 35 metastatic BRAF^V600E-mutant melanoma patients.

SUBMITTER: Barcelo C 

PROVIDER: S-EPMC9470708 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature