Low-level whole-brain radiation enhances theranostic potential of single-domain antibody fragments for human epidermal growth factor receptor type 2 (HER2)-positive brain metastases.

ABSTRACT:

Background

Single-domain antibody fragments (aka V_HH, ~ 13 kDa) are promising delivery systems for brain tumor theranostics; however, achieving efficient delivery of V_HH to intracranial lesions remains challenging due to the tumor-brain barrier. Here, we evaluate low-dose whole-brain irradiation as a strategy to increase the delivery of an anti- human epidermal growth factor receptor type 2 (HER2) V_HH to breast cancer-derived intracranial tumors in mice.

Methods

Mice with intracranial HER2-positive BT474BrM3 tumors received 10-Gy fractionated cranial irradiation and were evaluated by noninvasive imaging. Anti-HER2 V_HH 5F7 was labeled with ¹⁸F, administered intravenously to irradiated mice and controls, and PET/CT imaging

SUBMITTER: Procissi D 

PROVIDER: S-EPMC9476215 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature