Dataset Information

Comparative transcriptome and co-expression analysis reveal key genes involved in leaf margin serration in Perilla frutescens.

ABSTRACT:

Objective

In this study, we aimed to identify the genes involved in leaf margin serration in Perilla frutescens. P. frutescens (Family: Lamiaceae) is widely grown in Asian countries. Perilla leaf is the medicinal part stipulated in the Chinese Pharmacopoeia. There are mainly two types of perilla leaves: one with serrated leaf margin which is the phenotype described in the pharmacopoeia and the other with smooth leaf margin.

Methods

Transcriptome sequencing, co-expression analysis, and qRT-PCR analysis of six perilla tissues sampled from two different phenotypes (serrated and smooth leaves) were performed.

Results

Forty-three differentially expressed genes (DEGs), which may potentially regulate leaf shape, were identified through de novo transcriptome sequencing between the two groups. Genes involved in leaf shape regulation were identified. Simultaneously, we validated five DEGs by qRT-PCR, and the results were consistent with the transcriptome data. In addition, 1186 transcription factors (TFs) belonging to 45 TF families were identified. Moreover, the co-expression network of DEGs was constructed.

Conclusion

The study identified the key genes that control leaf shape by comparing the transcriptomes. Our findings also provide basic data for further exploring P. frutescens, which can help study the mechanism of leaf shape development and molecular breeding.

SUBMITTER: Shen Q

PROVIDER: S-EPMC9476768 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Comparative transcriptome and co-expression analysis reveal key genes involved in leaf margin serration in <i>Perilla frutescens</i>.

Shen Qi Q Zhang Dong D Zhang Tian-Yuan TY Xu Yang-Yang YY Zhao De-Gang DG

Chinese herbal medicines 20200620 3

<h4>Objective</h4>In this study, we aimed to identify the genes involved in leaf margin serration in <i>Perilla frutescens. P. frutescens</i> (Family: Lamiaceae) is widely grown in Asian countries. Perilla leaf is the medicinal part stipulated in the <i>Chinese Pharmacopoeia</i>. There are mainly two types of perilla leaves: one with serrated leaf margin which is the phenotype described in the pharmacopoeia and the other with smooth leaf margin.<h4>Methods</h4>Transcriptome sequencing, co-expres ...[more]

PMID: 36119006

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