NMRpQuant: an automated software for large scale urinary total protein quantification by one-dimensional 1H NMR profiles.

ABSTRACT:

Summary

1H nuclear magnetic resonance (NMR) spectroscopy is an established bioanalytical technology for metabolic profiling of biofluids in both clinical and large-scale population screening applications. Recently, urinary protein quantification has been demonstrated using the same 1D 1H NMR experimental data captured for metabolic profiling. Here, we introduce NMRpQuant, a freely available platform that builds on these findings with both novel and further optimized computational NMR approaches for rigorous, automated protein urine quantification. The results are validated by interlaboratory comparisons, demonstrating agreement with clinical/biochemical methodologies, pointing at a ready-to-use tool for routine protein urinalyses.

Availability and implementation

NMRpQuant wa

SUBMITTER: Takis PG 

PROVIDER: S-EPMC9477529 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature