Dataset Information

Prevalence of thrombophilia-associated genetic risk factors in blood donors of a regional hospital in southern Brazil.

ABSTRACT:

Introduction

Thromboembolic events occur due to an imbalance in the hemostasis and some factors associated with this condition can be inherited. In order to evaluate the frequency of genotypes considered to be common hereditary risk factors for thrombophilia associated with venous thrombosis (g.1691G > A and g.20210G > A) and hyperhomocysteinemia (g.677C > T and g.1298A > C), samples from voluntary healthy blood donors at the Hospital de Clínicas de Porto Alegre were tested.

Methods

We examined 325 blood samples from blood donors collected from October 2017 to July 2018. Blood was collected on filter paper and the DNA was extracted for single nucleotide polymorphisms (SNPs) analysis using the qualitative real time polymerase chain reaction.

Results

The calculated frequencies of each genetic variant in heterozygosity were 4% for the FV gene (g.1691G > A), 4% for the F2 gene (g.20210G > A) and 42% and 39% for methylenetetrahydrofolate reductase (MTHFR), g.677C > T and g.1298A > C, respectively. Only the genetic variants of MTHFR were found in homozygosity, with frequencies of 14% and 6% (g.677C > T and g.1298A > C), respectively.

Discussion

Altogether, these results describe the frequencies of genetic variants associated with venous thrombosis and hyperhomocysteinemia in the analyzed group and are important to enhance our current knowledge about the genetic profiles of Brazilian blood donors.

SUBMITTER: Dick-Guareschi J

PROVIDER: S-EPMC9477773 | biostudies-literature | 2022 Jul-Sep

REPOSITORIES: biostudies-literature

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Publications

Prevalence of thrombophilia-associated genetic risk factors in blood donors of a regional hospital in southern Brazil.

Dick-Guareschi Jéssica J Fontana Juliana Cristine JC Sanseverino Maria Teresa Vieira MTV Kubaski Francyne F Sekine Leo L Mesquita Nanci Félix NF Onsten Tor Gunnar Hugo TGH Leistner-Segal Sandra S

Hematology, transfusion and cell therapy 20210316 3

<h4>Introduction</h4>Thromboembolic events occur due to an imbalance in the hemostasis and some factors associated with this condition can be inherited. In order to evaluate the frequency of genotypes considered to be common hereditary risk factors for thrombophilia associated with venous thrombosis (g.1691G > A and g.20210G > A) and hyperhomocysteinemia (g.677C > T and g.1298A > C), samples from voluntary healthy blood donors at the Hospital de Clínicas de Porto Alegre were tested.<h4>Methods</ ...[more]

PMID: 33775585

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Project description:BackgroundHuman hepatitis is an inflammation of the liver brought on by the DNA virus known as the hepatitis B virus (HBV). Around the world, 240 million people are thought to have HBV in a chronic state. The prevalence of viral hepatitis is extremely high in Africa. Prior reports from various regions of the nation have indicated a variable prevalence of HBV infection.ObjectiveTo estimate the prevalence of HBV infections and associated factors among blood donors in the Hossana blood bank catchment area in August 2023.MethodsA community-based cross-sectional study design was conducted among 546 blood donors in the Hossana blood bank catchment area from August 22 to September 22, 2023. Study participants were included using the system established by the blood bank. Both Binary and multivariable logistic regression analysis was conducted. The association of variables was declared at p-value < 0.05 and strength of association was presented using AOR with a corresponding 95% CI. The adequacy of the model was checked by Hosmer-Lemeshow test.Resultsa total of 546 participant's data was analyzed. The prevalence of HBV among blood donors in the Hossana blood bank catchment area was 7.88 with 95% CI [5.76-10.46]. No participants received post-donation counseling. Having multiple sexual partners (AOR = 5.18, 95% CI: 1.32-20.36), Sharing of sharp material (AOR = 3.66, 95% CI: 1.32-10.13) and tooth extraction (AOR = 5.55, 95% CI: 2.08-14.78) were identified as factors associated with HBV positivity.ConclusionIn this study, the prevalence of hepatitis B virus was 7.88, which is intermediate. Multiple sexual partners, sharing of sharp material, and tooth extraction were related to the hepatitis b virus. Though post-donation counseling is important for the control and prevention of hepatitis b virus infection, it has been neglected since the establishment of the blood bank we strongly recommend counseling regular donors.Clinical trial numberNot applicable.

Dataset Information

Prevalence of thrombophilia-associated genetic risk factors in blood donors of a regional hospital in southern Brazil.

Introduction

Methods

Results

Discussion

Publications

Prevalence of thrombophilia-associated genetic risk factors in blood donors of a regional hospital in southern Brazil.

Similar Datasets

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