An in vivo transfection system for inducible gene expression and gene silencing in murine hepatocytes.

ABSTRACT:

Background

Hydrodynamic tail vein injection (HTVI) of transposon-based integration vectors is an established system for stably transfecting mouse hepatocytes in vivo that has been successfully employed to study key questions in liver biology and cancer. Refining the vectors for transposon-mediated hepatocyte transfection will further expand the range of applications of this technique in liver research. In the present study, we report an advanced transposon-based system for manipulating gene expression in hepatocytes in vivo.

Methods

Transposon-based vector constructs were generated to enable the constitutive expression of inducible Cre recombinase (CreER) together with tetracycline-inducible transgene or miR-small hairpin RNA (shRNA) expression (Tet-ON system). Transposon an

SUBMITTER: Hubner EK 

PROVIDER: S-EPMC9481923 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature