Detection of m⁶A RNA modifications at single-nucleotide resolution using m⁶A-selective allyl chemical labeling and sequencing.

ABSTRACT: As the most abundant internal mRNA modification, N⁶-methyladenosine (m⁶A) was involved in almost all the aspects of RNA metabolism. Here, we introduce our protocol for m⁶A-SAC-seq, which enables the whole transcriptome-wide mapping of m⁶A RNA modification at single-nucleotide resolution with stoichiometry information. m⁶A-SAC-seq relies on selective allyl labeling of m⁶A by specific methyltransferase and chemical treatment that introduce mutation upon reverse transcription. The technique only requires ∼30 ng of input RNA. For complete details on the use and execution of this protocol, please refer to Hu et al. (2022).

SUBMITTER: Peng Y 

PROVIDER: S-EPMC9485529 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature