CRISPRtracrRNA: robust approach for CRISPR tracrRNA detection.

ABSTRACT:

Motivation

The CRISPR-Cas9 system is a Type II CRISPR system that has rapidly become the most versatile and widespread tool for genome engineering. It consists of two components, the Cas9 effector protein, and a single guide RNA that combines the spacer (for identifying the target) with the tracrRNA, a trans-activating small RNA required for both crRNA maturation and interference. While there are well-established methods for screening Cas effector proteins and CRISPR arrays, the detection of tracrRNA remains the bottleneck in detecting Class 2 CRISPR systems.

Results

We introduce a new pipeline CRISPRtracrRNA for screening and evaluation of tracrRNA candidates in genomes. This pipeline combines evidence from different components of the Cas9-sgRNA complex. The core is a newly

SUBMITTER: Mitrofanov A 

PROVIDER: S-EPMC9486595 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature