Intact HIV Proviruses Persist in the Brain Despite Viral Suppression with ART.

ABSTRACT:

Objective

Human immunodeficiency virus (HIV) persistence in blood and tissue reservoirs, including the brain, is a major barrier to HIV cure and possible cause of comorbid disease. However, the size and replication competent nature of the central nervous system (CNS) reservoir is unclear. Here, we used the intact proviral DNA assay (IPDA) to provide the first quantitative assessment of the intact and defective HIV reservoir in the brain of people with HIV (PWH).

Methods

Total, intact, and defective HIV proviruses were measured in autopsy frontal lobe tissue from viremic (n = 18) or virologically suppressed (n = 12) PWH. Total or intact/defective proviruses were measured by detection of HIV pol or the IPDA, respectively, through use of droplet digital polymerase chain reactio

SUBMITTER: Cochrane CR 

PROVIDER: S-EPMC9489665 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature