Long-primed germinal centres with enduring affinity maturation and clonal migration.

ABSTRACT: Germinal centres are the engines of antibody evolution. Here, using human immunodeficiency virus (HIV) Env protein immunogen priming in rhesus monkeys followed by a long period without further immunization, we demonstrate germinal centre B (B_GC) cells that last for at least 6 months. A 186-fold increase in B_GC cells was present by week 10 compared with conventional immunization. Single-cell transcriptional profiling showed that both light- and dark-zone germinal centre states were sustained. Antibody somatic hypermutation of B_GC cells continued to accumulate throughout the 29-week priming period, with evidence of selective pressure. Env-binding B_GC cells were still 49-fold above baseline at 29 weeks, which suggests that they could remain active f

SUBMITTER: Lee JH 

PROVIDER: S-EPMC9491273 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature