Distinct architectural requirements for the parS centromeric sequence of the pSM19035 plasmid partition machinery.

ABSTRACT: Three-component ParABS partition systems ensure stable inheritance of many bacterial chromosomes and low-copy-number plasmids. ParA localizes to the nucleoid through its ATP-dependent nonspecific DNA-binding activity, whereas centromere-like parS-DNA and ParB form partition complexes that activate ParA-ATPase to drive the system dynamics. The essential parS sequence arrangements vary among ParABS systems, reflecting the architectural diversity of their partition complexes. Here, we focus on the pSM19035 plasmid partition system that uses a ParB_pSM of the ribbon-helix-helix (RHH) family. We show that parS_pSM with four or more contiguous ParB_pSM-binding sequence repeats is required to assemble a stable ParA_pSM-ParB_pSM

SUBMITTER: Volante A 

PROVIDER: S-EPMC9499535 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature