Dataset Information

Quantitative Proteomics and Functional Characterization Reveal That Glutathione Peroxidases Act as Important Antioxidant Regulators in Mulberry Response to Drought Stress.

ABSTRACT: Mulberry (Morus alba L.) has been an economically important food crop for the domesticated silkworm, Bombyx mori, in China for more than 5000 years. However, little is known about the mechanism underlying mulberry response to environmental stress. In this study, quantitative proteomics was applied to elucidate the molecular mechanism of drought response in mulberry. A total of 604 differentially expressed proteins (DEPs) were identified via LC-MS/MS. The proteomic profiles associated with antioxidant enzymes, especially five glutathione peroxidase (GPX) isoforms, as a scavenger of reactive oxygen species (ROS), were systematically increased in the drought-stressed mulberry. This was further confirmed by gene expression and enzymatic activity. Furthermore, overexpression of the GPX isoforms led to enhancements in both antioxidant system and ROS-scavenging capacity, and greater tolerance to drought stress in transgenic plants. Taken together, these results indicated that GPX-based antioxidant enzymes play an important role in modulating mulberry response to drought stress, and higher levels of GPX can improve drought tolerance through enhancing the capacity of the antioxidant system for ROS scavenging.

SUBMITTER: Zhang M

PROVIDER: S-EPMC9500794 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Quantitative Proteomics and Functional Characterization Reveal That Glutathione Peroxidases Act as Important Antioxidant Regulators in Mulberry Response to Drought Stress.

Zhang Minjuan M Li Wenqiang W Li Shuaijun S Gao Junru J Gan Tiantian T Li Qinying Q Bao Lijun L Jiao Feng F Su Chao C Qian Yonghua Y

Plants (Basel, Switzerland) 20220908 18

Mulberry (<i>Morus alba</i> L.) has been an economically important food crop for the domesticated silkworm, <i>Bombyx mori</i>, in China for more than 5000 years. However, little is known about the mechanism underlying mulberry response to environmental stress. In this study, quantitative proteomics was applied to elucidate the molecular mechanism of drought response in mulberry. A total of 604 differentially expressed proteins (DEPs) were identified via LC-MS/MS. The proteomic profiles associat ...[more]

PMID: 36145752

Similar Datasets

Project description:Desiccation tolerance is a complex phenomenon that depends on the regulated expression of numerous genes during dehydration and subsequent rehydration. Our previous study identified a chloroplast drought-induced stress protein (MaCDSP32) in mulberry, a thioredoxin (Trx) that is upregulated under drought conditions and is likely to confer drought tolerance to transgenic plants. Mulberry (Morus spp.) is an ecologically and economically important perennial woody plant that is widely used in forest management to combat desertification. However, its stress tolerance physiology is not well understood. In this study, the functions of MaCDSP32 gene were investigated. The expression of MaCDSP32 exhibited a circadian rhythm and was induced by mild and severe water deficits. Under abiotic stress, MaCDSP32-overexpressing plants exhibited increased stress sensitivity with lower water retention capacity and more severe lipid peroxidation than the wild-type (WT) plants. Furthermore, the activity of superoxide dismutase (SOD), the contents of proline and soluble sugars and the expression of stress-related transcription factors were lower in the MaCDSP32-overexpressing plants than in the WT plants. However, the MaCDSP32-overexpressing lines exhibited stronger recovery capability after rewatering post-drought. Moreover, the SOD enzyme activity, proline content, and soluble sugar content were higher in the transgenic plants after rewatering than in the WT plants. The production of the reactive oxygen species (ROS) H2O2 and O2 - was significantly lower in the transgenic plants than in the WT plants. In addition, under abiotic stress, the MaCDSP32-overexpressing lines exhibited improved seed germination and seedling growth, these effects were regulated by a positive redox reaction involving MaCDSP32 and one of its targets. In summary, this study indicated that MaCDSP32 from mulberry regulates plant drought tolerance and ROS homeostasis mainly by controlling SOD enzyme activity and proline and soluble sugar concentrations and that this control might trigger the stress response during seed germination and plant growth. Overall, MaCDSP32 exerts pleiotropic effects on the stress response and stress recovery in plants.

Dataset Information

Quantitative Proteomics and Functional Characterization Reveal That Glutathione Peroxidases Act as Important Antioxidant Regulators in Mulberry Response to Drought Stress.

Publications

Quantitative Proteomics and Functional Characterization Reveal That Glutathione Peroxidases Act as Important Antioxidant Regulators in Mulberry Response to Drought Stress.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets