The Effect of Anti-Inflammatory Topical Ophthalmic Treatments on In Vitro Corneal Epithelial Cells.

ABSTRACT:

Purpose

To compare the effect of three commonly prescribed anti-inflammatory eye drops on corneal epithelial cells in vitro.

Methods

Three different lines of human corneal epithelial cells were tested: primary cells cultured from donor tissue, commercially available primary cells, and immortalized cells. Cells were seeded on 96-well plates and treated with the following eye drops: cyclosporine 0.05%, lifitegrast 5%, and tacrolimus 0.03% or 0.1%. Exposure times tested were 30 seconds, 1 minute, 2 minutes, 1 hour, 2 hours, 4 hours, and 24 hours. Brightfield images and viability assays were analyzed 48 to 72 hours after the initiation of treatments. At least five replicates were tested per drug and time exposure.

Results

Commercially obtained primary cells showed reduced

SUBMITTER: Sella R 

PROVIDER: S-EPMC9513739 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature