Adam10-dependent Notch signaling establishes dental epithelial cell boundaries required for enamel formation.

ABSTRACT: The disintegrin and metalloproteinase Adam10 is a membrane-bound sheddase that regulates Notch signaling and ensures epidermal integrity. To address the function of Adam10 in the continuously growing incisors, we used Keratin14 ^Cre/+;Adam10 ^fl/fl transgenic mice, in which Adam10 is conditionally deleted in the dental epithelium. Keratin14 ^Cre/+;Adam10 ^fl/fl mice exhibited severe abnormalities, including defective enamel formation reminiscent of human enamel pathologies. Histological analyses of mutant incisors revealed absence of stratum intermedium, and severe disorganization of enamel-secreting ameloblasts. In situ hybridization and immunostaining analyses in the Keratin14 ^Cre/+;Adam10 ^<

SUBMITTER: Mitsiadis TA 

PROVIDER: S-EPMC9526176 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature