Cell-surface Labeling via Bioorthogonal Host-Guest Chemistry.

ABSTRACT: The widespread adoption of the bioorthogonal chemical reporter strategy revolutionized chemical biology. However, its translation to living mammals has been challenging, due to the size/stability properties of the chemical reporter group and/or the reaction kinetics of the labeling step. While developing new bioorthogonal reactions has been the traditional approach to optimizing the bioorthogonal chemical reporter strategy, here we present a different avenue, leveraging intermolecular interactions, to create bioorthogonal host-guest pairs. This approach, deemed "bioorthogonal complexation, does not rely on activated functional groups or second-order rate constants. We utilize the cucurbit[7]uril (CB[7]) scaffold to showcase bioorthogonal complexation and determine that medium-affinity (

SUBMITTER: Kataki-Anastasakou A 

PROVIDER: S-EPMC9527085 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature