Pygopus2 ameliorates mesenteric adipocyte poor differentiation to alleviate Crohn's disease -like colitis via the Axin2/GSK3β pathway.

ABSTRACT:

Objectives

Crohn's disease (CD) mesenteric adipose tissue (MAT) inflammation affects enteritis through the interaction between the mesentery and intestine, and we previously found that poorly differentiated mesenteric adipocytes were related to its inflammatory features. Pygopus2 (Pygo2) is a key negative regulator of adipocyte differentiation. We aimed to determine whether Pygo2 participates in CD mesenteric lesions and whether Pygo2 knockdown would be beneficial in a CD model (Il-10^-/- mice).

Methods

Pygo2 expression in MAT from control and CD patients and Il-10^-/- mice was measured by immunohistochemistry. Lentiviral transfection was used to regulate Pygo2 expression in Il-10^-/- mice, and the effects on mesenteric adipocyte differentiation

SUBMITTER: Li J 

PROVIDER: S-EPMC9528773 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature