Tracking Regulatory T Cell Development in the Thymus Using Single-Cell RNA Sequencing/TCR Sequencing.

ABSTRACT: Recent studies have demonstrated that regulatory T cells (T_regs) develop in the thymus via two pathways involving distinct T_reg progenitors (T_regP): CD25⁺FOXP3^- (CD25⁺ T_regP) and CD25^-FOXP3^lo (FOXP3^lo T_regP) T_reg progenitors. To examine this process in more detail, we carried out single-cell RNA sequencing (scRNA-Seq) and TCR-Seq on sorted murine CD4⁺CD8⁺ double-positive (DP) thymocytes, CD4⁺ single-positive (CD4SP) thymocytes, CD25⁺FOXP3^-CD73^- T_regP, CD25^-FOXP3^loCD73^- T_regP, newly generated mature CD25⁺FOXP3⁺CD73^-

SUBMITTER: Owen DL 

PROVIDER: S-EPMC9529998 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature