Complex genomic patterns of abasic sites in mammalian DNA revealed by a high-resolution SSiNGLe-AP method.

ABSTRACT: DNA damage plays a critical role in biology and diseases; however, how different types of DNA lesions affect cellular functions is far from clear mostly due to the paucity of high-resolution methods that can map their locations in complex genomes, such as those of mammals. Here, we present the development and validation of SSiNGLe-AP method, which can map a common type of DNA damage, abasic (AP) sites, in a genome-wide and high-resolution manner. We apply this method to six different tissues of mice with different ages and human cancer cell lines. We find a nonrandom distribution of AP sites in the mammalian genome that exhibits dynamic enrichment at specific genomic locations, including single-nucleotide hotspots, and is significantly influenced by gene expression, age and tissue type in

SUBMITTER: Cai Y 

PROVIDER: S-EPMC9534904 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature