A modification of nested PCR method for detection of Enterocytozoon hepatopenaei (EHP) in giant freshwater prawn Macrobrachium rosenbergii.

ABSTRACT: The microsporidian Enterocytozoon hepatopenaei (EHP) has become a critical threat to the global shrimp aquaculture industry, thus necessitating early detection by screening. Development of a rapid and accurate assay is crucial both for the active surveillance and for the assessment of shrimp with EHP infection. In the present study, a distinct strain of E. hepatopenaei (EHP _Mr ) was found in Macrobrachium rosenbergii. The SWP1 gene analysis revealed it was a new genotype that differed with the common strain isolated from the Litopenaeus vannamei (EHP _Lv ). A nested SWP-PCR method was modified to fix the bug that the original inner primers could not recognize the EHP _Mr strain. The redesigned inner prim

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9538563 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature