The combinatory effect of scaffold topography and culture condition: an approach to nucleus pulposus tissue engineering.

ABSTRACT: Scaffold topography and culture medium conditions for human wharton's jelly mesenchymal stem cells (hWJ-MSC) are critical components of the approach to nucleus pulposus (NP) tissue engineering.

Aim

To evaluate the silk fibroin (SF) scaffold topography analysis (optimal thickness and pore diameter) and to determine culture medium conditions for the growth and differentiation of hWJ-MSC.

Method

hWJ-MSCs were seeded into different thicknesses and pore size diameters and grown in different concentrations of glucose, platelet rich plasma (PRP) and oxygen. The cell-seeded scaffold was evaluated for cell attachment, growth and differentiation potency.

Results & discussion

The results indicated that SF scaffold with a minimum thickness 3.5 mm and pore diameter of 500 μm with c

SUBMITTER: Vanawati N 

PROVIDER: S-EPMC9540240 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature