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Transesterification of Non-Activated Esters Promoted by Small Molecules Mimicking the Active Site of Hydrolases.

ABSTRACT: The synthesis of small molecules able to mimic the active site of hydrolytic enzymes has been largely pursued in recent decades. The high reaction rates and specificity shown by natural hydrolases present an attractive target, and yet the preparation of suitable small-molecule mimics remains challenging, requiring activated substrates to achieve productive outcomes. Here we present small synthetic artificial enzymes which mimic the catalytic site and the oxyanion hole of chymotrypsin and N-terminal hydrolases and are able to perform, for the first time, the transesterification of a non-activated ester such as ethyl acetate with methanol under mild and neutral reaction conditions.

SUBMITTER: Garrido-Gonzalez JJ

PROVIDER: S-EPMC9544131 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Transesterification of Non-Activated Esters Promoted by Small Molecules Mimicking the Active Site of Hydrolases.

Garrido-González José J JJ Sánchez-Santos Estela E Habib Asmaa A Cuevas Ferreras Ángel V ÁV Sanz Francisca F Morán Joaquín R JR Fuentes de Arriba Ángel L ÁL

Angewandte Chemie (International ed. in English) 20220607 35

The synthesis of small molecules able to mimic the active site of hydrolytic enzymes has been largely pursued in recent decades. The high reaction rates and specificity shown by natural hydrolases present an attractive target, and yet the preparation of suitable small-molecule mimics remains challenging, requiring activated substrates to achieve productive outcomes. Here we present small synthetic artificial enzymes which mimic the catalytic site and the oxyanion hole of chymotrypsin and N-termi ...[more]

PMID: 35580193

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