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Contactless crystallization method of protein by a magnetic force booster.

ABSTRACT: We developed a new type of compact magnetic force booster by which we succeeded in crystallizing proteins (hen egg white lysozyme) while making them levitate in a solution without contacting the container. This technique is noteworthy in the practical merit that we could control the growth of crystals from the initial stage of nucleation in a magnetic field of merely a few Tesla. The shape of the booster was designed in accordance with the dynamical stability against external forces acting on the crystals. Under a stable condition, the crystals condensed spherically, and formed a "shell shaped" crystallization with a hollow interior. Our magnetic force booster has the potential for use in innovation, especially in the field of protein crystal engineering.

SUBMITTER: Maki S

PROVIDER: S-EPMC9568516 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Contactless crystallization method of protein by a magnetic force booster.

Maki Syou S Hagiwara Masayuki M

Scientific reports 20221014 1

We developed a new type of compact magnetic force booster by which we succeeded in crystallizing proteins (hen egg white lysozyme) while making them levitate in a solution without contacting the container. This technique is noteworthy in the practical merit that we could control the growth of crystals from the initial stage of nucleation in a magnetic field of merely a few Tesla. The shape of the booster was designed in accordance with the dynamical stability against external forces acting on th ...[more]

PMID: 36241850

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