Catechin-Albumin Conjugates: Enhanced Antioxidant Capacity and Anticancer Effects.

ABSTRACT: (+)-Catechin conjugated with human serum albumin (CT-HSA) was prepared and evaluated as a drug carrier bearing anticancer effects. It was found that 2.4 mol of CT was conjugate to 1 mol HSA. The CT-HSA has an antioxidant capacity of about 3.3 times the amount of CT in the conjugate. Intracellular incorporation of the CT-HSA was analyzed by fluorescence-activated cell sorting (FACS) and confocal laser scanning microscopy (CLSM) measurements using fluorescein isothiocyanate (FITC)-labelled CT-HSA. The results indicated that the FITC-labelled CT-HSA was incorporated into HeLa cells in a concentration-dependent manner. The CT-HSA enhanced the binding of anticancer drugs (5-fluorouracil (5-Fu) and mitomycin C (MMC)) comparing with HSA, and the CT-HSA mixed with 5-Fu or MMC decreased significant

SUBMITTER: Ooya T 

PROVIDER: S-EPMC9569216 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature