Real time monitoring of cold Ca²⁺ dependent transcription and its modulation by NCX inhibitors.

ABSTRACT: Real-time monitoring of cellular temperature responses is an important technique in thermal biology and drug development. Recent study identified that Na⁺/Ca²⁺ exchanger (NCX)-dependent Ca²⁺ influx transduces cold signals to circadian clock in mammalian cultured cells. The finding raised an idea that cellular responses to the cold signals can be analyzed by monitoring of clock gene expression. We found that Per1 and Per2 were up-regulated after culture at 27 °C compared to 37 °C in Rat-1 fibroblasts. In order to monitor cold-Ca²⁺-dependent transcription in living cells, we developed a luciferase-based real-time reporting system by using Per1 promoter, Per2 promoter, Ca²⁺/cAMP-response elements (CRE) or NFAT-binding elements. We found t

SUBMITTER: Wang HT 

PROVIDER: S-EPMC9569354 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature