Pancreatic Cancer Cells Induce MicroRNA Deregulation in Platelets.

ABSTRACT: Pancreatic cancer is a pathology with a high mortality rate since it is detected at advanced stages, so the search for early-stage diagnostic biomarkers is essential. Liquid biopsies are currently being explored for this purpose and educated platelets are a good candidate, since they are known to present a bidirectional interaction with tumor cells. In this work, we analyzed the effects of platelets on cancer cells' viability, as determined by MTT, migration using transwell assays, clonogenicity in soft agar and stemness by dilution assays and stem markers' expression. We found that the co-culture of platelets and pancreatic cancer cells increased the proliferation and migration capacity of BXCP3 cells, augmented clonogenicity and induced higher levels of Nanog, Sox2 and Oct4 expression. A

SUBMITTER: Diaz-Blancas JY 

PROVIDER: S-EPMC9569638 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature