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Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells.


ABSTRACT: Protein-protein interaction studies provide valuable insights into cellular signaling. Brassinosteroid (BR) signaling is initiated by the hormone-binding receptor Brassinosteroid Insensitive 1 (BRI1) and its co-receptor BRI1 Associated Kinase 1 (BAK1). BRI1 and BAK1 were shown to interact independently with the Receptor-Like Protein 44 (RLP44), which is implicated in BRI1/BAK1-dependent cell wall integrity perception. To demonstrate the proposed complex formation of BRI1, BAK1 and RLP44, we established three-fluorophore intensity-based spectral Förster resonance energy transfer (FRET) and FRET-fluorescence lifetime imaging microscopy (FLIM) for living plant cells. Our evidence indicates that RLP44, BRI1 and BAK1 form a ternary complex in a distinct plasma membrane nanodomain. In contrast, although the immune receptor Flagellin Sensing 2 (FLS2) also forms a heteromer with BAK1, the FLS2/BAK1 complexes are localized to other nanodomains. In conclusion, both three-fluorophore FRET approaches provide a feasible basis for studying the in vivo interaction and sub-compartmentalization of proteins in great detail.

SUBMITTER: Glockner N 

PROVIDER: S-EPMC9571070 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells.

Glöckner Nina N   Zur Oven-Krockhaus Sven S   Rohr Leander L   Wackenhut Frank F   Burmeister Moritz M   Wanke Friederike F   Holzwart Eleonore E   Meixner Alfred J AJ   Wolf Sebastian S   Harter Klaus K  

Plants (Basel, Switzerland) 20221006 19


Protein-protein interaction studies provide valuable insights into cellular signaling. Brassinosteroid (BR) signaling is initiated by the hormone-binding receptor Brassinosteroid Insensitive 1 (BRI1) and its co-receptor BRI1 Associated Kinase 1 (BAK1). BRI1 and BAK1 were shown to interact independently with the Receptor-Like Protein 44 (RLP44), which is implicated in BRI1/BAK1-dependent cell wall integrity perception. To demonstrate the proposed complex formation of BRI1, BAK1 and RLP44, we esta  ...[more]

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