Dataset Information

Exploring Active Ingredients, Beneficial Effects, and Potential Mechanism of Allium tenuissimum L. Flower for Treating T2DM Mice Based on Network Pharmacology and Gut Microbiota.

ABSTRACT: Forty compounds were isolated and characterized from A. tenuissimum flower. Among them, twelve flavonoids showed higher α-glucosidase inhibition activities in vitro than acarbose, especially kaempferol. The molecular docking results showed that the binding of kaempferol to α-glucosidase (GAA) could reduce the hydrolysis of substrates by GAA and reduce the glucose produced by hydrolysis, thus exhibiting α-glucosidase inhibition activities. The in vivo experiment results showed that flavonoids-rich A. tenuissimum flower could decrease blood glucose and reduce lipid accumulation. The protein expression levels of RAC-alpha serine/threonine-protein kinase (AKT1), peroxisome proliferator activated receptor gamma (PPARG), and prostaglandin G/H synthase 2 (PTGS2) in liver tissue were increased. In addition, the Firmicutes/Bacteroidetes (F/B) ratio was increased, the level of gut probiotics Bifidobacterium was increased, and the levels of Enterobacteriaceae and Staphylococcus were decreased. The carbohydrate metabolism, lipid metabolism, and other pathways related to type 2 diabetes mellitus were activated. This study indicating flavonoids-rich A. tenuissimum flower could improve glycolipid metabolic disorders and inflammation in diabetic mice by modulating the protein expression and gut microbiota.

SUBMITTER: Zhang SS

PROVIDER: S-EPMC9571170 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Exploring Active Ingredients, Beneficial Effects, and Potential Mechanism of <i>Allium tenuissimum</i> L. Flower for Treating T2DM Mice Based on Network Pharmacology and Gut Microbiota.

Zhang Shan-Shan SS Hou Yu-Fei YF Liu Shao-Jing SJ Guo Sen S Ho Chi-Tang CT Bai Nai-Sheng NS

Nutrients 20220925 19

Forty compounds were isolated and characterized from <i>A. tenuissimum</i> flower. Among them, twelve flavonoids showed higher α-glucosidase inhibition activities in vitro than acarbose, especially kaempferol. The molecular docking results showed that the binding of kaempferol to α-glucosidase (GAA) could reduce the hydrolysis of substrates by GAA and reduce the glucose produced by hydrolysis, thus exhibiting α-glucosidase inhibition activities. The in vivo experiment results showed that flavono ...[more]

PMID: 36235633

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Dataset Information

Exploring Active Ingredients, Beneficial Effects, and Potential Mechanism of Allium tenuissimum L. Flower for Treating T2DM Mice Based on Network Pharmacology and Gut Microbiota.

Publications

Exploring Active Ingredients, Beneficial Effects, and Potential Mechanism of <i>Allium tenuissimum</i> L. Flower for Treating T2DM Mice Based on Network Pharmacology and Gut Microbiota.

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