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Site-Specific Radioiodination of Oligonucleotides with a Phenolic Element in a Programmable Approach.

ABSTRACT: Radioiodination of oligonucleotides provides an extra modality for nucleic acid-based theranostics with potential applications. Herein, we report the design and synthesis of a phosphoramidite embedded with a phenolic moiety and demonstrate that oligonucleotides can be readily functionalized with phenol as a precursor by general DNA synthesis. It was identified that the introduction of the precursor does not block the specificity of an aptamer, and the radioiodination is applicable to both DNA and RNA oligonucleotides in a site-specific approach with a commercial kit.

SUBMITTER: Zhao H

PROVIDER: S-EPMC9571250 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Site-Specific Radioiodination of Oligonucleotides with a Phenolic Element in a Programmable Approach.

Zhao Haitao H Qin Yu Y Liu Dunfang D Geng Xinyao X Wang Cheng C Ding Ding D Ding Xuan X Xia Qian Q Liu Jianjun J Wang Ruowen R Tan Weihong W

Molecules (Basel, Switzerland) 20220923 19

Radioiodination of oligonucleotides provides an extra modality for nucleic acid-based theranostics with potential applications. Herein, we report the design and synthesis of a phosphoramidite embedded with a phenolic moiety and demonstrate that oligonucleotides can be readily functionalized with phenol as a precursor by general DNA synthesis. It was identified that the introduction of the precursor does not block the specificity of an aptamer, and the radioiodination is applicable to both DNA an ...[more]

PMID: 36234794

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