Single-cell RNA-sequencing technology demonstrates the heterogeneity between aged prostate peripheral and transitional zone.

ABSTRACT:

Background

Identifying cellular and functional heterogeneity within aged prostate is critical for understanding the spatial distribution of prostate diseases.

Methods

Aged human prostate peripheral zone (PZ) and transitional zone (TZ) tissues were used for single-cell RNA-sequencing. Results were validated by immunofluorescence staining.

Results

We found that club/hillock epithelial cells, compared with other epithelial cells, had significantly higher NOTCH signaling activity and expressed higher levels of neuro-stems but lower androgen-related genes. These cells were primarily found in the TZ and provided a stem-like niche around the proximal prostate ducts. Significant heterogeneity was observed in the aged luminal population. A novel TFF3+ luminal subtype with elev

SUBMITTER: Yan Q 

PROVIDER: S-EPMC9574492 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature