Asymbiotic in vitro seed germination, in vitro seedling development, and ex vitro acclimatization of Spiranthes

ABSTRACT: Abstract

Premise

Reproducible seed propagation and production protocols were developed for Spiranthes and related taxa to facilitate ex situ conservation practices.

Methods and Results

Spiranthes seeds were scarified for 3‐ and 10‐min intervals in 10% sodium hypochlorite solution, then cultured on three seed germination media. After germination, seedlings were given one of the three photoperiod treatments, and then planted in one of four greenhouse substrates. Seed germination ranged from 0% to 90% and occurred on all three media only after the 3‐min scarification. Seedlings in the 24/0‐h light/dark and 16/8‐h light/dark photoperiods on P723 medium had significantly higher fresh weight than those in the dark treatment group. Ex vitro survival ranged from 55% to 95% across

SUBMITTER: Zale P 

PROVIDER: S-EPMC9575095 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature