Tnpo3 enables EBF1 function in conditions of antagonistic Notch signaling.

ABSTRACT: Transcription factor EBF1 (early B cell factor 1) acts as a key regulator of B cell specification. The transcriptional network in which EBF1 operates has been extensively studied; however, the regulation of EBF1 function remains poorly defined. By mass spectrometric analysis of proteins associated with endogenous EBF1 in pro-B cells, we identified the nuclear import receptor Transportin-3 (Tnpo3) and found that it interacts with the immunoglobulin-like fold domain of EBF1. We delineated glutamic acid 271 of EBF1 as a critical residue for the association with Tnpo3. EBF1^E271A showed normal nuclear localization; however, it had an impaired B cell programming ability in conditions of Notch signaling, as determined by retroviral transduction of Ebf1 ^-/- progenitors

SUBMITTER: Bayer M 

PROVIDER: S-EPMC9575695 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature