Selective Isotope Labeling and LC-Photo-CIDNP Enable NMR Spectroscopy at Low-Nanomolar Concentration.

ABSTRACT: NMR spectroscopy is a powerful tool to investigate molecular structure and dynamics. The poor sensitivity of this technique, however, limits its ability to tackle questions requiring dilute samples. Low-concentration photochemically induced dynamic nuclear polarization (LC-photo-CIDNP) is an optically enhanced NMR technology capable of addressing the above challenge by increasing the detection limit of aromatic amino acids in solution up to 1000-fold, either in isolation or within proteins. Here, we show that the absence of NMR-active nuclei close to a magnetically active site of interest (e.g., the structurally diagnostic ¹H^α-¹³C^α pair of amino acids) is expected to significantly increase LC-photo-CIDNP hyperpolarization. Then, we exploit the sp

SUBMITTER: Yang H 

PROVIDER: S-EPMC9577358 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature