Costimulators expressed on human endothelial cells modulate antigen-dependent recruitment of circulating T lymphocytes.

ABSTRACT: Endothelial cells (ECs) can present antigens to circulating effector memory T cells (T_EM) and to regulatory T cells (T regs), triggering antigen-specific extravasation at specific sites where foreign antigens are introduced, e.g. by infection or transplantation. We model human antigen-induced transendothelial migration (TEM) using presentation of superantigen by cultured human dermal microvascular (HDM)ECs to isolated resting human peripheral blood T cell subpopulations or to T effector cells activated in vitro. T cell receptor (TCR)-mediated cytokine synthesis, a common assay of T cell activation by antigen, is modulated by antigen-independent signals provided by various positive or negative costimulator proteins (the latter known as checkpoint inhibitors) expressed by a

SUBMITTER: Manes TD 

PROVIDER: S-EPMC9582530 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature