Eicosanoids in the pancreatic tumor microenvironment - a multicellular, multifaceted progression.

ABSTRACT:

Background and aims

Eicosanoids, oxidized fatty acids that serve as cell-signaling molecules, have been broadly implicated in tumorigenesis. Here, we aimed to identify eicosanoids associated with pancreatic tumorigenesis and the cell types responsible for their synthesis.

Methods

We profiled normal pancreas and pancreatic ductal adenocarcinoma (PDAC) in mouse models and patient samples using mass spectrometry. We interrogated RNA sequencing datasets for eicosanoid synthase or receptor expression. Findings were confirmed by immunostaining.

Results

In murine models, we identified elevated levels of PGD₂, prostacyclin, and thromboxanes in neoplasia while PGE₂, 12-HHTre, HETEs, and HDoHEs are elevated specifically in tumors. Analysis of scRNA-seq dat

SUBMITTER: Gubbala VB 

PROVIDER: S-EPMC9583893 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature