Dataset Information

The relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses.

ABSTRACT: Infection with Gasterophilus intestinalis (botfly) larvae often occurs in horses. The aim of the study was to isolate the larvae of G. intestinalis and evaluate the serum and salivary humoral immune response using self-developed ELISA in G. intestinalis infected horses. Blood serum or saliva samples were taken from 125 infected horses and 54 uninfected slaughtered horses. The antigens from G. intestinalis larvae were used for development of ELISA in order to evaluate the intensity of G. intestinalis IgG, IgM, and IgA antibody reactivity in the serum or saliva of naturally infected horses and horses without larvae in the gastrointestinal tract (control group). Serum antibodies against second and third larvae's stadium antigens reacted significantly more intensively in infected than in healthy horses in IgG (p ≤ 0.001; p ≤ 0.05, respectively) and IgA (p ≤ 0.05;p ≤ 0.001, respectively) classes. Salivary IgG and IgA specific's antibody reactivity was significantly higher in horses with moderate (p ≤ 0.01) and severe infection (p ≤ 0.001) compared to the healthy horses. The determination of the G. intestinalis IgG and IgA antibody activity in saliva and serum may be used for detecting horses moderately and severely infested with larvae.

SUBMITTER: Pawlas-Opiela M

PROVIDER: S-EPMC9585061 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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The relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses.

Pawlas-Opiela Monika M Jawor Paulina P Galli Józef J Zak-Bochenek Agnieszka A Gorczykowski Michał M Galli Joanna J Sołtysiak Zenon Z Stefaniak Tadeusz T

Scientific reports 20221020 1

Infection with Gasterophilus intestinalis (botfly) larvae often occurs in horses. The aim of the study was to isolate the larvae of G. intestinalis and evaluate the serum and salivary humoral immune response using self-developed ELISA in G. intestinalis infected horses. Blood serum or saliva samples were taken from 125 infected horses and 54 uninfected slaughtered horses. The antigens from G. intestinalis larvae were used for development of ELISA in order to evaluate the intensity of G. intestin ...[more]

PMID: 36266409

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Project description:Work-related stress in police officers has been recognized as a risk factor for mental and physical illness, compromising work performance and public safety. While genetic, individual and environmental factors may contribute to stress, basic pathophysiological mechanisms are still not completely understood. Previous studies have shown the potential of microRNAs (miRNAs) as biomarkers and/or mediator for stress responses. In this study, we aimed at identifying possible dysregulation of salivary miRNAs associated with work-related stress in police officers. The study was conducted in a cohort composed of police officers in service at the mobile unit of Genoa in 2009, whose response to occupational stress has been continuously monitored since 2009 using the Demand-Control and the Effort-Reward Imbalance models. 113 male police officers in service in 2022 were recruited and divided in three groups according to their response to stress (low, intermediate and high stress response). miRNAs were extracted from saliva samples and analyzed by small RNA sequencing followed by an integrated bioinformatics/biostatistics analysis. The high stress response group was characterized by 18 differentially expressed miRNAs compared with the low stress response group, with four miRNAs (miR-10400-5p, miR-1290, miR-6074-5p, and miR-9902) at higher levels and fourteen miRNAs (miR-203a-3p, miR-7-5p, miR-143-3p, miR-181a-5p, miR-142-5p, miR-223-5p, let-7f-5p, let-7g-5p, miR-26b-5p, miR-27a-3p, miR-30e-5p, miR-26a-5p, miR-142-3p, and miR-21-5p) at lower levels. We also discovered sets of miRNAs that exhibited dynamic expression changes in response to intermediate and low stress. Functional enrichment analysis identified processes related to apoptosis, cellular response to stress, negative regulation of gene expression, responses to abiotic stimuli, positive regulation of catabolism as commonly regulated by the modulated miRNAs. Work-related stress response was associated with specific salivary miRNA expression patterns, with a general decrease of miRNA levels in the highly distressed police officers. These results suggest salivary miRNA profiling as a non-invasive and useful biomarker of stress and further characterize the link between chronic psychosocial stress and cellular and behavioral response mechanisms.

Dataset Information

The relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses.

Publications

The relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses.

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