Dataset Information

The Construction and Analysis of a ceRNA Network Related to Salt-Sensitivity Hypertensives.

ABSTRACT:

Background

Salt-sensitivity hypertensives (SSH) are an independent risk factor for cardiovascular disease. However, the mechanism of SSH is not clear. This study is aimed at constructing a competing endogenous RNA (ceRNA) network related to SSH.

Methods

Data sets were collected from the Gene Expression Omnibus database (GEO) to extract data on salt sensitivity RNA of patients with or without hypertensives in GSE135111. Firstly, we analyzed differentially expressed genes (DEGs, log2FC ≥ 0.5 and P < 0.05) and differentially expressed lncRNAs (DELs, log2FC ≥1 and P<0.05) between SSH and salt-sensitive normotension (SSN). Then, the gene ontology (GO), KEGG pathway enrichment analysis, and PPI network construction of DEGs were performed, and the hub genes in the PPI network by cytoHubba (12 methods) were screened out. Finally, a ceRNA network was constructed based on lncRNA-miRNA-mRNA pairs and hub genes.

Results

163 DEGs and 65 DELs were screened out. The GO and KEGG pathway analyses of DEGs were mainly enriched in metabolism (e.g., insulin secretion and cellular response to glucagon stimulus and peptidyl-tyrosine dephosphorylation,) and plasma membrane signaling (e.g., cell adhesion and chemical synaptic transmission and integral component of membrane). Additionally, a ceRNA network, including 1 mRNA (EGLN3), 2 miRNAs (hsa-miR-17-5p and hsa-miR-20b-5p), and 1 lncRNA (C1orf143) was successfully constructed.

Conclusions

In conclusion, the proposed ceRNA network may help elucidate the regulatory mechanism by which lncRNAs function as ceRNAs and contribute to the pathogenesis of SSH. Importantly, candidate lncRNAs, miRNAs, and mRNAs can be further evaluated as a potential therapeutic targets for SSH.

SUBMITTER: Liu XJ

PROVIDER: S-EPMC9586768 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Publications

The Construction and Analysis of a ceRNA Network Related to Salt-Sensitivity Hypertensives.

Liu Xiu-Juan XJ Yin Hong-Lin HL Li Yan Y Hao Hao H Liu Yang Y Zhao Quan-Lin QL

BioMed research international 20221014

<h4>Background</h4>Salt-sensitivity hypertensives (SSH) are an independent risk factor for cardiovascular disease. However, the mechanism of SSH is not clear. This study is aimed at constructing a competing endogenous RNA (ceRNA) network related to SSH.<h4>Methods</h4>Data sets were collected from the Gene Expression Omnibus database (GEO) to extract data on salt sensitivity RNA of patients with or without hypertensives in GSE135111. Firstly, we analyzed differentially expressed genes (DEGs, log ...[more]

PMID: 36277897

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Dataset Information

The Construction and Analysis of a ceRNA Network Related to Salt-Sensitivity Hypertensives.

Background

Methods

Results

Conclusions

Publications

The Construction and Analysis of a ceRNA Network Related to Salt-Sensitivity Hypertensives.

Similar Datasets

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