Production and biochemical characterization of partially purified cellulase-free, thermo-acidophilic endoxylanase from Lysinibacillus fusiformis strain TB7 using kolanut husk as feedstock.

ABSTRACT: Xylanases have become very important enzymes in many industrial processes for the valorization of xylan-rich lignocellulosic wastes. Here, some physicochemical and kinetic properties of a purified endoxylanase produced on kolanut husk-based medium by Lysinibacillus fusiformis are presented. The crude enzyme solution was first subjected to precipitation with solid ammonium sulphate and further purified on DEAE-Sephadex A-50 anion-exchange and Sephadex G-100 gel filtration columns chromatography prior to biochemical characterization. The purified endoxylanase was 21 kDa as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and was thermostable, exhibiting optimum activity at 60 °C and pH 5.0. The K _m and V _max

SUBMITTER: Omisore SO 

PROVIDER: S-EPMC9586915 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature