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Production and biochemical characterization of partially purified cellulase-free, thermo-acidophilic endoxylanase from Lysinibacillus fusiformis strain TB7 using kolanut husk as feedstock.

ABSTRACT: Xylanases have become very important enzymes in many industrial processes for the valorization of xylan-rich lignocellulosic wastes. Here, some physicochemical and kinetic properties of a purified endoxylanase produced on kolanut husk-based medium by Lysinibacillus fusiformis are presented. The crude enzyme solution was first subjected to precipitation with solid ammonium sulphate and further purified on DEAE-Sephadex A-50 anion-exchange and Sephadex G-100 gel filtration columns chromatography prior to biochemical characterization. The purified endoxylanase was 21 kDa as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and was thermostable, exhibiting optimum activity at 60 °C and pH 5.0. The K _m and V _max were respectively estimated to be 29.5 mg/ml and 125 μmol/min/ml using Birchwood xylan as substrate. Activity of the enzyme was enhanced by Na⁺, Ca²⁺, Mn²⁺, Mg²⁺ and K⁺ at concentration of 5 mM but inhibited by Hg²⁺, Cu²⁺, Pb²⁺, Fe³⁺, EDTA, SDS and Urea. The purified endoxylanase showed high hydrolytic activity on Birchwood xylan and kolanut husk but extremely poor or no activity on carboxymethyl cellulose, starch or pectin. This L. fusiformis strain TB7 endoxylanase has desirable properties useful for biotechnological applications in laundry, fuels, feeds, paper and pulp industries.

SUBMITTER: Omisore SO

PROVIDER: S-EPMC9586915 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Production and biochemical characterization of partially purified cellulase-free, thermo-acidophilic endoxylanase from <i>Lysinibacillus fusiformis</i> strain TB7 using kolanut husk as feedstock.

Omisore Suliat Olatidayo SO Fabunmi Temitope Bukola TB Ayodeji Adeyemi Oluwadare AO Olaniyi Oladipo Oladiti OO Arotupin Daniel Juwon DJ

Heliyon 20221014 10

Xylanases have become very important enzymes in many industrial processes for the valorization of xylan-rich lignocellulosic wastes. Here, some physicochemical and kinetic properties of a purified endoxylanase produced on kolanut husk-based medium by <i>Lysinibacillus fusiformis</i> are presented. The crude enzyme solution was first subjected to precipitation with solid ammonium sulphate and further purified on DEAE-Sephadex A-50 anion-exchange and Sephadex G-100 gel filtration columns chromatog ...[more]

PMID: 36281386

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Project description:In recent years, biofilms have become a central subject of research in the fields of microbiology, medicine, agriculture, and systems biology, among others. The sociomicrobiology of multispecies biofilms, however, is still poorly understood. Here, we report a screening system that allowed us to identify soil bacteria which induce architectural changes in biofilm colonies when cocultured with Bacillus subtilis We identified the soil bacterium Lysinibacillus fusiformis M5 as an inducer of wrinkle formation in B. subtilis colonies mediated by a diffusible signaling molecule. This compound was isolated by bioassay-guided chromatographic fractionation. The elicitor was identified to be the purine hypoxanthine using mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy. We show that the induction of wrinkle formation by hypoxanthine is not dependent on signal recognition by the histidine kinases KinA, KinB, KinC, and KinD, which are generally involved in phosphorylation of the master regulator Spo0A. Likewise, we show that hypoxanthine signaling does not induce the expression of biofilm matrix-related operons epsABCDEFGHIJKLMNO and tasA-sipW-tapA Finally, we demonstrate that the purine permease PbuO, but not PbuG, is necessary for hypoxanthine to induce an increase in wrinkle formation of B. subtilis biofilm colonies. Our results suggest that hypoxanthine-stimulated wrinkle development is not due to a direct induction of biofilm-related gene expression but rather is caused by the excess of hypoxanthine within B. subtilis cells, which may lead to cell stress and death.IMPORTANCE Biofilms are a bacterial lifestyle with high relevance regarding diverse human activities. Biofilms can be beneficial, for instance, in crop protection. In nature, biofilms are commonly found as multispecies communities displaying complex social behaviors and characteristics. The study of interspecies interactions will thus lead to a better understanding and use of biofilms as they occur outside laboratory conditions. Here, we present a screening method suitable for the identification of multispecies interactions and showcase L. fusiformis as a soil bacterium that is able to live alongside B. subtilis and modify the architecture of its biofilms.

Dataset Information

Production and biochemical characterization of partially purified cellulase-free, thermo-acidophilic endoxylanase from Lysinibacillus fusiformis strain TB7 using kolanut husk as feedstock.

Publications

Production and biochemical characterization of partially purified cellulase-free, thermo-acidophilic endoxylanase from <i>Lysinibacillus fusiformis</i> strain TB7 using kolanut husk as feedstock.

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