Memory CD4⁺ T cells that co-express PD1 and CTLA4 have reduced response to activating stimuli facilitating HIV latency.

ABSTRACT: Programmed cell death 1 (PD1) and cytotoxic T lymphocyte-associated protein 4 (CTLA4) suppress CD4⁺ T cell activation and may promote latent HIV infection. By performing leukapheresis (n = 21) and lymph node biopsies (n = 8) in people with HIV on antiretroviral therapy (ART) and sorting memory CD4⁺ T cells into subsets based on PD1/CTLA4 expression, we investigate the role of PD1 and CTLA 4 in HIV persistence. We show that double-positive (PD1⁺CTLA4⁺) cells in blood contain more HIV DNA compared with double-negative (PD1^-CTLA4^-) cells but still have a lower proportion of cells producing multiply spliced HIV RNA after stimulation as well as reduced upregulation of T cell activation and proliferation markers. Transcriptomics a

SUBMITTER: Rasmussen TA 

PROVIDER: S-EPMC9589005 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature