Target engagement of ginsenosides in mild cognitive impairment using mass spectrometry-based drug affinity responsive target stability.

ABSTRACT:

Background

Mild cognitive impairment (MCI) is a transitional condition between normality and dementia. Ginseng is known to have effects on attenuating cognitive deficits in neurogenerative diseases. Ginsenosides are the main bioactive component of ginseng, and their protein targets have not been fully understood. Furthermore, no thorough analysis is reported in ginsenoside-related protein targets in MCI.

Methods

The candidate protein targets of ginsenosides in brain tissues were identified by drug affinity responsive target stability (DARTS) coupled with label-free liquid chromatography-mass spectrometry (LC-MS) analysis. Network pharmacology approach was used to collect the therapeutic targets for MCI. Based on the above-mentioned overlapping targets, we built up a protein-

SUBMITTER: Zhu Z 

PROVIDER: S-EPMC9597435 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature