DEPs Induce Local Ige Class Switching Independent of Their Ability to Stimulate iBALT de Novo Formation.

ABSTRACT:

Background

Diesel exhaust particles (DEPs) are leading to a general increase in atopic diseases worldwide. However, it is still unknown whether DEPs induce systemic B-cell IgE class switching in secondary lymphoid organs or locally in the lungs in inducible bronchus-associated lymphoid tissue (iBALT). The aim of this work was to identify the exact site of DEP-mediated B-cell IgE class switching and pro-allergic antibody production.

Methods

We immunized BALB/c mice with different OVA doses (0.3 and 30 µg) intranasally in the presence and absence of two types of DEPs, SRM1650B and SRM2786. We used low (30 µg) and high (150 µg) DEP doses.

Results

Only a high DEP dose induced IgE production, regardless of the particle type. Local IgE class switching was stimulated upon tr

SUBMITTER: Chudakov DB 

PROVIDER: S-EPMC9603618 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature