Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus.

ABSTRACT: The 2022 multi-country monkeypox (mpox) outbreak concurrent with the ongoing COVID-19 pandemic has further highlighted the need for genomic surveillance and rapid pathogen whole genome sequencing. While metagenomic sequencing approaches have been used to sequence many of the early mpox infections, these methods are resource intensive and require samples with high viral DNA concentrations. Given the atypical clinical presentation of cases associated with the outbreak and uncertainty regarding viral load across both the course of infection and anatomical body sites, there was an urgent need for a more sensitive and broadly applicable sequencing approach. Highly multiplexed amplicon-based sequencing (PrimalSeq) was initially developed for sequencing of Zika virus, and later adapted as the mai

SUBMITTER: Chen NFG 

PROVIDER: S-EPMC9603838 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature