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Increased CD4⁺CD8⁺ Double Positive T Cells during Hantaan Virus Infection.

ABSTRACT: Hantaan virus (HTNV) infection causes an epidemic of hemorrhagic fever with renal syndrome (HFRS) mainly in Asia. It is well known that T cells mediated anti-viral immune response. Although previous studies showed that double positive T (DP T) cells, a little portion of T lymphocytes, were involved in adaptive immune response during virus infection, their kinetic changes and roles in HTNV infection have not yet been explored. In this study, we characterized DP T cells from HFRS patients based on flow cytometry data combined with scRNA-seq data. We showed that HTNV infection caused the upregulation of DP T cells in the peripheral blood, which were correlated with disease stage. The scRNA-seq data clustered DP T cells, unraveled their gene expression profile, and estimated the ordering of these cells. The production of granzyme B and CD107a from DP T cells and the abundant TCR distribution indicated the anti-viral property of DP T cells. In conclusion, this study identified, for the first time, an accumulation of DP T cells in the peripheral blood of HFRS patients and suggested these DP T cells belonging to CD8⁺T cells lineage. The DP T cells shared the similar characteristics with cytotoxic T cells (CTL) and exerted an anti-viral role in HFRS.

SUBMITTER: Zhang H

PROVIDER: S-EPMC9611689 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Increased CD4<sup>+</sup>CD8<sup>+</sup> Double Positive T Cells during Hantaan Virus Infection.

Zhang Huiyuan H Wang Yazhen Y Ma Ying Y Tang Kang K Zhang Chunmei C Wang Meng M Zhang Xiyue X Xue Manling M Jia Xiaozhou X Hu Haifeng H Li Na N Zhuang Ran R Jin Boquan B Chen Lihua L Zhang Yun Y Zhang Yusi Y

Viruses 20221013 10

Hantaan virus (HTNV) infection causes an epidemic of hemorrhagic fever with renal syndrome (HFRS) mainly in Asia. It is well known that T cells mediated anti-viral immune response. Although previous studies showed that double positive T (DP T) cells, a little portion of T lymphocytes, were involved in adaptive immune response during virus infection, their kinetic changes and roles in HTNV infection have not yet been explored. In this study, we characterized DP T cells from HFRS patients based on ...[more]

PMID: 36298798

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Project description:BackgroundCD4+/CD8+ double positive (DP) T cells have been described in healthy individuals as well as in patients with autoimmune and chronic infectious diseases. In chronic viral infections, this cell subset has effector memory phenotype and displays antigen specificity. No previous studies of double positive T cells in parasite infections have been carried out.Methodology/principal findingsSeventeen chronic chagasic patients (7 asymptomatic and 10 symptomatic) and 24 non-infected donors, including 12 healthy and 12 with non-chagasic cardiomyopathy donors were analyzed. Peripheral blood was stained for CD3, CD4, CD8, HLA-DR and CD38, and lymphocytes for intracellular perforin. Antigen specificity was assessed using HLA*A2 tetramers loaded with T. cruzi K1 or influenza virus epitopes. Surface expression of CD107 and intracellular IFN-γ production were determined in K1-specific DP T cells from 11 chagasic donors. Heart tissue from a chronic chagasic patient was stained for both CD8 and CD4 by immunochemistry. Chagasic patients showed higher frequencies of DP T cells (2.1% ± 0.9) compared with healthy (1.1% ± 0.5) and non-chagasic cardiomyopathy (1.2% ± 0.4) donors. DP T cells from Chagasic patients also expressed more HLA-DR, CD38 and perforin and had higher frequencies of T. cruzi K1-specific cells. IFN-γ production in K1-specific cells was higher in asymptomatic patients after polyclonal stimulation, while these cells tended to degranulate more in symptomatic donors. Immunochemistry revealed that double positive T cells infiltrate the cardiac tissue of a chagasic donor.ConclusionsChagasic patients have higher percentages of circulating double positive T cells expressing activation markers, potential effector molecules and greater class I antigenic specificity against T. cruzi. Although K1 tetramer positive DP T cell produced little IFN-γ, they displayed degranulation activity that was increased in symptomatic patients. Moreover, K1-specific DP T cells can migrate to the heart tissue.

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Increased CD4⁺CD8⁺ Double Positive T Cells during Hantaan Virus Infection.

Publications

Increased CD4<sup>+</sup>CD8<sup>+</sup> Double Positive T Cells during Hantaan Virus Infection.

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Dataset Information

Increased CD4+CD8+ Double Positive T Cells during Hantaan Virus Infection.

Publications

Increased CD4<sup>+</sup>CD8<sup>+</sup> Double Positive T Cells during Hantaan Virus Infection.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

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Increased CD4⁺CD8⁺ Double Positive T Cells during Hantaan Virus Infection.