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Cost-efficient boundary-free surface patterning achieves high effective-throughput of time-lapse microscopy experiments.

ABSTRACT: Time-lapse microscopy plays critical roles in the studies of cellular dynamics. However, setting up a time-lapse movie experiments is not only laborious but also with low output, mainly due to the cell-losing problem (i.e., cells moving out of limited field of view), especially in a long-time recording. To overcome this issue, we have designed a cost-efficient way that enables cell patterning on the imaging surfaces without any physical boundaries. Using mouse embryonic stem cells as an example system, we have demonstrated that our boundary-free patterned surface solves the cell-losing problem without disturbing their cellular phenotype. Statistically, the presented system increases the effective-throughput of time-lapse microscopy experiments by an order of magnitude.

SUBMITTER: Liang G

PROVIDER: S-EPMC9612557 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Cost-efficient boundary-free surface patterning achieves high effective-throughput of time-lapse microscopy experiments.

Liang Guohao G Yin Hong H Allard Jun J Ding Fangyuan F

PloS one 20221027 10

Time-lapse microscopy plays critical roles in the studies of cellular dynamics. However, setting up a time-lapse movie experiments is not only laborious but also with low output, mainly due to the cell-losing problem (i.e., cells moving out of limited field of view), especially in a long-time recording. To overcome this issue, we have designed a cost-efficient way that enables cell patterning on the imaging surfaces without any physical boundaries. Using mouse embryonic stem cells as an example ...[more]

PMID: 36301804

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