Integrated metabolomic and transcriptomic analyses of the synergistic effect of polymyxin-rifampicin combination against Pseudomonas aeruginosa.

ABSTRACT:

Background

Understanding the mechanism of antimicrobial action is critical for improving antibiotic therapy. For the first time, we integrated correlative metabolomics and transcriptomics of Pseudomonas aeruginosa to elucidate the mechanism of synergistic killing of polymyxin-rifampicin combination.

Methods

Liquid chromatography-mass spectrometry and RNA-seq analyses were conducted to identify the significant changes in the metabolome and transcriptome of P. aeruginosa PAO1 after exposure to polymyxin B (1 mg/L) and rifampicin (2 mg/L) alone, or in combination over 24 h. A genome-scale metabolic network was employed for integrative analysis.

Results

In the first 4-h treatment, polymyxin B monotherapy induced significant lipid perturbations, predominantly to fatty acid

SUBMITTER: Mahamad Maifiah MH 

PROVIDER: S-EPMC9618192 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature