Moringa oleifera leaf extract induces osteogenic-like differentiation of human osteosarcoma SaOS2 cells.

ABSTRACT:

Introduction

Moringa oleifera is known as a 'natural nutrition of the tropics' because it provides vital nutritional supplements and a variety of pharmacological benefits. The focus of this study was to elucidate the dose dependent effects of Moringa oleifera leaf (MOL) extract on the growth of the human osteoblast-like osteosarcoma SaOS-2 cell line and primary osteoblast cells.

Methods

Trypan blue & tetrazolium assay, intracellular ROS generation, chromatin condensation, cell cycle analysis, alkaline phosphatase (ALP), mineralization, and osteogenic gene expression were tested on both treated and untreated osteosarcoma SaOS-2 cells.

Results

As revealed by cell viability assay, growth activity was observed at concentrations 25 and 50 μg/mL of MOL extract

SUBMITTER: Khan MI 

PROVIDER: S-EPMC9618397 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature