A New Auto-RPA-Fluorescence Detection Platform for SARS-CoV-2.

ABSTRACT:

Objective

The outbreak of COVID-19 caused by SARS-CoV-2 has led to a serious worldwide pandemic. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR)-based methods were recommended for routine detection of SARS-CoV-2 RNA. Because the reaction time and analytical sensitivity of qRT-PCR limits the diagnosis of SARS-CoV-2, development of a quick process of SARS-CoV-2 detection technology with high analytical sensitivity remains urgent.

Methods

We combined isothermal amplification and fluorescence detection technology to develop a new auto-recombinase polymerase amplification (RPA)-fluorescence platform that could be used in the diagnosis of SARS-CoV-2.

Results

By optimization of primers and probes, the RPA platform could detect SARS-CoV-2 nucleotides wi

SUBMITTER: Tian J 

PROVIDER: S-EPMC9619631 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature