Chitooligosaccharide prevents vascular endothelial cell apoptosis by attenuation of endoplasmic reticulum stress via suppression of oxidative stress through Nrf2-SOD1 up-regulation.

ABSTRACT:

Context

Endoplasmic reticulum (ER) stress contributes to endothelium pathological conditions. Chitooligosaccharides (COS) have health benefits, but their effect on endothelial cells is unknown. We demonstrate for the first time a protective effect of COS against ER-induced endothelial cell damage.

Objective

To evaluate the protective effect of COS on ER stress-induced apoptosis in endothelial cells.

Material and methods

Endothelial (EA.hy926) cells were pre-treated with COS (250 or 500 μg/mL) for 24 h, and then treated with 0.16 μg/mL of Tg for 24 h and compared to the untreated control. Apoptosis and necrosis were detected by Annexin V-FITC/propidium iodide co-staining. Reactive oxygen species (ROS) were measured with the DCFH₂-DA and DHE probes. The prote

SUBMITTER: Ei ZZ 

PROVIDER: S-EPMC9621211 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature