Prevalence and distribution of ampc beta-lactamase producing escherichia coli and klebsiella pneumoniae isolates obtained from urine samples at a tertiary care hospital in the caribbean.

ABSTRACT:

Introduction

The aim of this study was to investigate the prevalence and distribution of AmpC beta-lactamases (BLs) in uropathogens (E. coli and K. pneumoniae) at the University Hospital of the West Indies Jamaica (UHWI).

Method

De-duplicated consecutive urine samples, collected from January to March 2020 at the UHWI, were analyzed. Screening and phenotypic confirmatory tests were conducted using resistance to cefoxitin and the Disc Approximation Test (DAT) respectively, for isolates of interest. Multiplex PCR was performed on cefoxitin resistant (CR) isolates for the detection of bla _CIT, bla _MOX, bla _FOX, bla _ACC, and bla _DHA genes. Whole genome sequencing (WGS) was used

SUBMITTER: Thoms Rodriguez CA 

PROVIDER: S-EPMC9623010 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature