De novo transcriptome based insights into secondary metabolite biosynthesis in Malaxis acuminata (Jeevak)-A therapeutically important orchid.

ABSTRACT: Malaxis acuminata D. Don [=Crepidium acuminatum (D. Don) Szlach.] is an endangered medicinal orchid of the Ashtvarga group of plants in Ayurveda (Indian system of traditional medicine). Using a combination of aromatic cytokinin [meta-Topolin (mT)], plant biostimulant (chitosan), auxin [indole-3-butyric acid (IBA)], and a phenolic elicitor [phloroglucinol (PG)], plants of M. acuminata were regenerated in vitro for mass multiplication. The present research reveals the first-ever transcriptome of M. acuminata. A total of 43,111 transcripts encoding 23,951 unigenes were assembled de novo from a total of 815.02 million reads obtained from leaf and pseudobulb of in vitro raised M. acuminata. Expression analysis o

SUBMITTER: Bhattacharyya P 

PROVIDER: S-EPMC9623264 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature