Vaccines against the original strain of SARS-CoV-2 provide T cell memory to the B.1.1.529 variant.

ABSTRACT:

Background

The SARS-CoV-2 variant B.1.1.529 potentially escapes immunity from vaccination via a heavily mutated Spike protein. Here, we analyzed whether T cell memory towards the B.1.1.529 Spike protein is present in individuals who received two or three doses of vaccines designed against the original Wuhan strain of SARS-CoV-2.

Methods

PBMCs were isolated from two- and three-times vaccinated study participants and incubated in vitro with peptide pools of the Spike protein derived from sequences of the original Wuhan or the B.1.1.529 strains of SARS-CoV-2. Activated antigen-specific T cells were detected by flow cytometry. In silico analyses with NetMHCpan and NetMHCIIpan were used to determine differences in MHC class presentation between the original strain and the B.1.1.5

SUBMITTER: Dornte C 

PROVIDER: S-EPMC9629766 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature